Figure 8. Effect of spiked CS-A on binding to L-selectin.

Splenocytes (5.0×10⁶ cells/ml) were cocultured with OVA (final concentration 100 μg/ml) and/or the rat anti-mouse CD62L antibody (final concentration 2 μg/ml) in triplicate at 37 °C in a CO₂ incubator for 3 days. The colleted cells were then staining with FITC-labelled anti anti-mouse CD62L antibody (MEL-14) and analysed on FACSCalibur™ flow cytometer (Becton Dickinson). (A) control, (B) after culture with CS, and (C) after culture with CS and the rat anti-mouse CD62L antibody.