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Photoacoustic (PA)-based ablation-induced lesion boundary detection pipeline. (a) Multi-wavelength PA images were unmixed based on the reference spectra of ablated and non-ablated tissue. (b) Necrotic mapping was computed from the ratio between ablated and non-ablated tissue intensity and filtered based on spectral noise. (c) Lesion area was thresholded, and linear depth compensation was applied. (d) Binary lesion map was displayed based on the depth-corrected PA image.
