Figure 2. Relative quantification of IMPI cDNA in comparison with actin cDNA by real-time PCR.

(A) IMPI mRNA is up-regulated upon LPS stimulation, as demonstrated by the lower number of cycles needed to amplify IMPI. (B) PCR with β-actin-specific primers using the same cDNA preparations as in (A) indicates no difference in the total cDNA amount between the different probes.