Table 1. Comparison of obtained and expected molecular masses of native IMPI and rIMPIs.

The differences between the obtained and expected molecular masses are hypothetically due to distinct glycosylation in the Drosophila expression system. Therefore we include possible N- and O-glycosylation sites along the IMPI coding and vector-derived amino acid sequences

				Expected IMPI part			Expected vector part
	Molecular mass (Da)				Glycosylation sites			Glycosylation sites
Protein	Obtained	Expected	Difference	Mass (Da)	N	O	Mass (Da)	N	O
Native IMPI	8364
rIMPI-1	13367	12474	893	7755	1	3	4737	−	7
rIMPI-1*	9418	8509	909	7755	1	3	772	−	1
rIMPI-2	14188	21256	−7068	16636	2	16	4638	1	7
rIMPI-2 after SPC	14188	12964	1224	8587	1	13	4395	1	6