Figure 2. Poly(ADP-ribosyl)ation of AP-2α prevents DNA binding.

EMSAs were performed with a ³²P-end-labelled double-stranded oligo containing an AP-2-binding site. Either 200 ng of recombinant AP-2α or the same amount of in vitro poly(ADP-ribosyl)ated protein was used in the binding assay. 3-AB or 6-Phen was added during the poly(ADP-ribosyl)ation as indicated. A single arrow indicates the AP-2α plus DNA complex and a double arrowhead the supershifted complex.