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. 2004 Aug 10;382(Pt 1):375–383. doi: 10.1042/BJ20040142

Figure 4. Co-localization of pECFP-S100P and pEYFP-S100A1.

Figure 4

MCF-7 cells were routinely cultured and co-transfected with expression plasmids encoding ECFP-S100A1 and S100P-EYFP. After 24 h, the images were taken under a confocal microscope. (A–D) Show the same field. S100P-EYFP, shown in pseudocolour green (A) is present in both nucleus and cytoplasm, but in some cells S100P-EYFP is present either in the nucleus or in the cytoplasm. In one of the cells shown, S100P-EYFP is concentrated around the peri-nuclear region (arrow). ECFP-S100A1, shown in pseudocolour red (B), distributes in an almost identical pattern with that of S100P-EYFP, irrespective of the overall pattern of localization in the cell. The images in (A, B) are superimposed (D), the yellow colour confirming the co-localization of S100P-EYFP and ECFP-S100A1. (C) Phase-contrast image of the same field.