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. 2004 Aug 10;382(Pt 1):375–383. doi: 10.1042/BJ20040142

Figure 5. Effect of S100A1 on the binding of S100P to immobilized rhC-MHC-IIA.

Figure 5

Purified rhC-MHC-IIA was immobilized on an aminosilane surface as described in the Experimental section. S100P and S100A1 were preincubated for various times at an equimolar ratio at room temperature or at 4 °C (A) or at various ratios for 60 min at room temperature (B). To detect the binding response, 2 μl of each mixture was added to rhC-MHC-IIA surface in a total volume of 30 μl [the final concentration of S100P protein in (A) was 25 nM and in (B) was 33 nM]. The extent of binding of each binding reaction was calculated using the Fastfit software. The means±S.E.M. was determined from at least four individual binding experiments. C (in A), 25 mM S100P only as a positive control.