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. 2004 Aug 10;382(Pt 1):385–392. doi: 10.1042/BJ20040065

Figure 3. Western blot analysis of EGFP-containing constructs expressed in HeLa cells.

Figure 3

Whole-cell extracts of HeLa cells expressing each EGFP-containing construct were separately resolved by SDS/PAGE and identified by Western blots with anti-EGFP antibody. The non-transfected HeLa cells were used as a negative control. Expressed EGFP of molecular mass 28 kDa was used as the marker indicated by the arrow. (A) Lanes: a, preOTCsp–EGFP-ER; b, preOTCsp–EGFP; c, EGFP; d, untransfected cells. (B) Lanes: a, preAIIsp–EGFP-ER; b, preAIIsp–EGFP; c, EGFP; d, untransfected cells. (C) Lanes: a, preOTCsp–EGFP–SKL; b, EGFP–SKL; c, EGFP; d, untransfected cells.