Table 1. Results of two-hybrid screen of human adult and foetal brain cDNA libraries using EPAC1 as bait.

	Screen 1 (human foetal brain)	Screen 2 (human adult brain)
No. of transformants	5000000	5000000
No. of His+ colonies	180	110
No. of LacZ+ colonies	120/180	90/110
No. of plasmids analysed by sequencing	30	38
No. of different clones present	3	7
Clone identities following BLAST search	Clone E4: NM_002373.3, human MAP1A	Clone A1: NP_503522.1, T-complex polypeptide 1/cpn60, required for assembly of microtubules
	Clone E8: BT008258.1, human prefoldin 5
	Clone E10: XM_040708.3, human KIAA1377 protein	Clone C2: NP_03249, keratin complex
		Clone D4: AY050668.1, human PKC-potentiated PP1
		Clone F1: AF300619, p32-RACK
		Clone F2: XM_030426.1, human hypoxia-inducible factor
		Clone F3: NM_002373.3, human MAP1A