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. 2004 Sep 7;382(Pt 3):811–819. doi: 10.1042/BJ20040808

Figure 7. Localization of endogenous Slc11a1.

Figure 7

Localization of endogenous wild-type and mutant Slc11a1 in bone marrow-derived macrophages. Day 10 macrophages from Slc11a1 mutant C57BL/10ScSn versus congenic Slc11a1 wild-type N20, B10.L-Lshr mice were activated for 24 h with interferon γ/lipopolysaccharide. After methanol fixation, wild-type (A, C) and mutant (B, D) macrophages were stained with a polyclonal anti-N-terminal Slc11a1 antibody (green), combined with either anti-Lamp1 (A, B) or anti-Pdi (C, D), both shown in red. Merged images are presented with yellow identifying the co-localization of wild-type Slc11a1 with Lamp1 and partial co-localization of mutant Slc11a1 with Pdi. Scale bar, 10 μm.