Figure 2. NAD⁺-induced rise in [Ca²⁺]_i depends on extracellular Ca²⁺.

(A) Monocytes were incubated with NAD⁺ (200 μM) in a Ca²⁺-containing or Ca²⁺-free solution. Results are means±S.E.M. (n=35–49 cells) of one out of three experiments. ***P<0.001 compared with control (Ca²⁺-containing solution); Student's t test. (B) Monocytes were pre-incubated with LaCl₃ (1 mM) or BaCl₂ (2 mM) for 30 min before NAD⁺ (200 μM) was added. Results are means±S.E.M. (n=20–55 cells) of one out of three experiments. ***P<0.001 compared with control (in the absence of LaCl₃ or BaCl₂); Student's t test.