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. 2004 Sep 7;382(Pt 3):857–865. doi: 10.1042/BJ20040916

Figure 1. PI 3-kinase products regulate membrane association of GFP–GRP1, but not GFP–Tiam1.

Figure 1

Transfected 1321N1 astrocytoma cells were fixed with paraformaldehyde, mounted with Mowiol, and imaged and deconvolved with Openlab software, as described in the Experimental section. Cells were transfected with GFP–GRP1 (i–iv) or GFP–C1199-Tiam1 (v–viii). Cells were left unstimulated [i, ii, v and vi; control (con)], stimulated with 1 μM IGF-1 for 10 min (iii, iv, vii and viii), and/or pre-treated with 100 nM wortmannin for 30 min (ii, iv, vi and viii), before being fixed. Results are representative of three different experiments.