Figure 6.

Expression of SNGs in iNeurons

(A) 193 SNGs are expressed during the transdifferentiation of HDFs to iNeurons. Querying the list differentially expressed genes identified in cells undergoing transdifferentiation at days 10, 20, and 26 reveals that 193 of the 516 SNG genes are upregulated in iNeurons and with expression >1 TPM in at least one time point analyzed, with 133 of these displaying TPM >5.

(B–D) Expression of the 193 SNGs during transdifferentiation of HDFs to iNeurons. (B) Heatmap shows expression of individual genes reported as TPM (log₁₀). (C) Violin plots show significant upregulation of the cohort of 193 SNGs. Expression is reported as the mean TPM from across all four replicates for each time point. Statistical analysis was determined by ordinary one-way ANOVA with Tukey’s multiple comparison test. ^∗∗∗∗p < 0.0001. (D) Categorization of the 193 genes as having expression within 0–1 TPM, 1–5 TPM, 5–10 TPM, and 10+ TPM, at each time point.

(E) Correlation of expression (TPM) between the 193 iNeuron expressed SNGs at day 26 transdifferentiation and mean expression (TPM) in the human adult frontal cortex.

(F) Comparison of expression of 100 of the 193 iNeuron-expressed SNGs with range of expression observed in the adult frontal cortex samples.

(G) Comparison of the isoform diversity between SNGs expressed in iNeurons to that of the adult frontal cortex. Adult frontal cortex expression data were extracted from the GTEx database Version 8.