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. 2004 Sep 7;382(Pt 3):905–912. doi: 10.1042/BJ20040911

Figure 5. Probe 7-P[14C]Man is a glycosyl donor photoinhibitor of M. smegmatis α(1→6)mannosyltransferase.

Figure 5

The indicated mannosylated lipid monophosphate substrates (100000 c.p.m., 140 pmol) in 0.25% CHAPS were mixed with a M. smegmatis membrane preparation and some were photolysed at 4 °C using a UV lamp (365 nm) for 90 min. The non-photolysed (NP) and photolysed (P) samples were then incubated at 37 °C for 1 h along with the synthetic Manp(1→6)Manp-O-C10:1 disaccharide acceptor and processed and subjected to TLC/autoradiography as described previously [23] to determine the extent of product formation {[14C]M-M2-O-C10:1, [14C]Manp-(1→6)Manp(1→6)Manp-O-C10:1}.