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. 2004 Sep 7;382(Pt 3):913–921. doi: 10.1042/BJ20040432

Figure 1. PGC-1α enhances the transcriptional activation of GAL4–FXR most effectively, among SRC1a, SRC1e, PGC-1α, PGC-1β, Tip60 and P/CAF, in a ligand-dependent manner.

Figure 1

(A) Transfections of COS-7 cells and measurements of luciferase activity were carried out as described in the Experimental section using pCMX-GAL4-FXR (200 ng), UASx4-tk Luc reporter construct (400 ng), co-activator expression construct (200 ng) and pEYFP-C1 (200 ng), in the absence (open bars) or presence (closed bars) of 100 μM CDCA. (B) The pCMX-flag-PGC-1α expression plasmid used for transfection was at amounts of between 0 and 200 ng, and similar experiments were performed. (C) The transfection was carried out using pCMX-GAL4-FXR (200 ng), UASx4-tk Luc reporter construct (400 ng) or pCMX-flag-PGC-1α (200 ng) in the presence of increasing amounts of CDCA from 0 to 200 μM. (D) Similar experiments were carried out in the presence of 100 μM CA (cholic acid), CDCA, DCA or LCA.