(A) Transfections and measurements of luciferase activities were carried out using pCMX-GAL4-FXR (100 ng), UASx4-tk Luc reporter construct (200 ng), pCMX-flag-PGC-1α (100 ng), pEYFP-C1 (100 ng) and increasing amounts of pCMX-SHP (30, 100 and 500 ng) in the presence of 100 μM CDCA. (B) Similar experiments were carried out using pCMX-FXR (100 ng), pCMX-RXRα (100 ng), pGL3-hSHP(569)-Luc (200 ng), pCMX-flag-PGC-1α (200 ng), pEYFP-C1 (100 ng) and increasing amounts of pCMX-SHP (100, 200 and 300 ng) in the presence of 1 μM 9-cis-retinoic acid and 100 μM CDCA.