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The experiments were carried out using pCMX-FXR (40 ng), pCMX-RXRα (40 ng), pEYFP-C1 (100 ng), pCMX-SHP (0 or 40 ng) and increasing amounts of pCMX-flag-PGC-1α (50, 100 and 300 ng) in the presence of 1 μM 9-cis-retinoic acid and 100 μM CDCA.
