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. 2024 Aug 7;96(33):13533–13541. doi: 10.1021/acs.analchem.4c02051

Figure 4.

Figure 4

GAPPIS MS3 data identified proteins with a significant MW shift. (a) Volcano plot with 102 proteins significantly shifted to lower MW (red), and 3 proteins significantly shifted to higher MW (blue). (b) Volcano plot of semitryptic peptides with K/R as the last amino acid residue cells with a significant increase (red) and decrease (blue) in the abundance ratio to fully tryptic peptide STS-treated HeLa cells. (c) Same for semitryptic peptides with K/R before the first amino acid residue. (d) Overlap of the GAPPIS-identified caspase 3 substrates with the three previous studies. (e) Preferred sequence motif for the cleavage sites in 24 semitryptic peptides.