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. 2004 Sep 7;382(Pt 3):995–1001. doi: 10.1042/BJ20040793

Table 2. MS analysis of N-glycans in the TKO worm.

The Table shows ratios of peak heights of mutant C. elegans N-glycans relative to peak heights of wild-type N-glycans after MALDI-TOF MS analysis. Normal structures which are absent in the mutant worms (ratio=0/X) are shown in bold type. Structures seen in the mutant but not in the wild-type worm (ratio=Y/0) are shown in italics. The hexose residues in Hex2–Hex9 are probably mannose residues, but the presence of some galactose cannot be ruled out [45,46]; Hex10 is presumably Glc1Man9. Abbreviations: F, L-fucose; Me, O-methyl. Hex3GlcNAc3 and Hex3GlcNAc4 probably represent truncated mono-antennary and bi-antennary complex N-glycans respectively.

Ratio of peak height
N-glycan Hex2 Hex3 Hex4 Hex5 Hex6 Hex7 Hex8 Hex9 Hex10
GlcNAc2 0/100 10/34 100/41 30/20 24/16 12/12 6/14 1/1
GlcNAc3 0/18
GlcNAc4 0/5
GlcNAc2F1 0/38 4/46 5/18 12/0 10/0 4/0 3/0
GlcNAc2F2 0/23 0/31 0/48 2/36 2/17 2/1 2/0
GlcNAc2F3 0/14 0/36 0/40 0/27 0/10 0/1
GlcNAc2F4 0/10 0/10 0/7 0/2
GlcNAc2F1Me 0/16 0/5
GlcNAc2F2Me 0/10 0/20 0/7
GlcNAc2F3Me 0/16 0/18 0/16 0/8 0/1
GlcNAc2F4Me 0/18 0/11 0/10 0/3