Table 3. Inhibition constants for 2′-AMP and 5′-AMP with respect to NADP⁺ in wild-type NADP⁺-ME and K435/6L mutant.

The values presented are the means±S.E.M. for at least three separate determinations.

	Ki (μM)
Enzyme	2′-AMP	5′-AMP
Wild-type	104.5±1.2	439.2±6.2
K435/6L	687.6±12.3	255.5±6.1
Wild-type/K435/6L	0.15	1.7