. 2024 Aug 6;63(16):2023–2029. doi: 10.1021/acs.biochem.4c00292

Table 3. Kinetic Parameters of KLK2 Hydrolysis of FRET Peptides Derived from the IL-10R2 Ectodomain Containing Arginine (R) and Lysine (K)^a.

fragment	position in IL-10R2	FRET peptide	k_cat (s^–1)	Km (μM)	k_cat/Km (mM s–1)
1	²⁶NVRM³⁰N	Abz-NVR↓^bMN-Q-EDDnp	1.28	0.94	1362
2	⁵⁸SYRI⁶²F	Abz-SYR↓^bIFQ-Q-EDDnp	7.3	0.95	7684
3	⁷⁹LSKYG⁸⁴D	Abz-LSKYGD-Q-EDDnp	no hydrolysis
4	⁸⁶TLRVRA⁹²E	Abz-TLR↓^bVR↓^bAE-Q-EDDnp	2.47	1.34	1843
5	¹²⁸HMRF¹³²L	Abz-HMR↓^bFL-Q-EDDnp	0.51	0.51	1000
6	¹⁷⁷VLRN¹⁸¹L	Abz-VLR↓^bNL-Q-EDDnp	1.61	0.39	4
7	¹⁹⁰QVRG¹⁹⁴F	Abz-QVR↓^bGF-Q-EDDnp	0.77	1.15	670
8	¹⁹⁵LPDRN²⁰⁰K	Abz-LPDRNK-Q-EDDnp	no hydrolysis

Complete ectodomain sequence of human IL-10R2 with highlighted arginines (R), one lysine (K), and the sequence ⁵⁸SYRI⁶²F. 1 MAWSLGSWLG GCLLVSALGM VPPPENVRMN SVNFKNILQW ESPAFAKGNL TFTAQYL⁵⁸SYR. 61 I⁶²FQDKCMNTT LTECDFSSLS KYGDHTLRVR AEFADEHSDW VNITFCPVDD TIIGPPGMQV. 121 EVLADSLHMR FLAPKIENEY ETWTMKNVYN SWTYNVQYWK NGTDEKFQIT PQYDFEVLRN. 181 LEPWTTYCVQ VRGF LPDRNK AGEWSEPVCE QTTHDETVPS.

↓ Represents the cleavage sites.

Table 3. Kinetic Parameters of KLK2 Hydrolysis of FRET Peptides Derived from the IL-10R2 Ectodomain Containing Arginine (R) and Lysine (K)a.

Table 3. Kinetic Parameters of KLK2 Hydrolysis of FRET Peptides Derived from the IL-10R2 Ectodomain Containing Arginine (R) and Lysine (K)^a.