. 2024 Aug 9;9(33):35703–35717. doi: 10.1021/acsomega.4c04100

Table 1. Primers’ Forward and Reverse Sequences with Their Optimized Annealing Temperature^a.

primer	primer sequence	Ta (°C)
MAOA	forward: 5-GCCAAGATTCACTTCAGACCAGAG-3	59
MAOA	reverse: 5-TGCTCCTCACACCAGTTCTTCTC-3	59
Bcl-2	forward: 5-TTGTGGCCTTCTTTGAGTTCGGTG-3	59
Bcl-2	reverse: 5-GGTGCCGGTTCAGGTACTCAGTCA-3	59
VEGF	forward: 5-CTACCTCCACCATGCCAAGT-3	59
VEGF	reverse: 5-GCAGTAGCTGCGCTGATAGA-3	59
GAPDH	forward: 5-ACAACTTTGGTATCGTGGAAGG-3	58
GAPDH	reverse: 5-GCCATCACGCCACAGTTTC-3	58

Ta: annealing temperature; MAOA: gene for monoamine oxidase-A enzyme; Bcl-2: gene for B-cell lymphoma 2; VEGF: gene for vascular endothelial growth factor; GAPDH: gene for glyceraldehyde 3-phosphate dehydrogenase.

Table 1. Primers’ Forward and Reverse Sequences with Their Optimized Annealing Temperaturea.

Table 1. Primers’ Forward and Reverse Sequences with Their Optimized Annealing Temperature^a.