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. 2024 Aug 22;57:56. doi: 10.1186/s40659-024-00537-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Layer expression of NMDARs in the hippocampus of APP/PS1 mice. (A-I) The hippocampal layer expression of the GluN1 protein was visualised using histoblots of horizontal brain sections at 1, 6 and 12 months of age in wild type and APP/PS1 mice using an affinity-purified anti-GluN1 antibody. Densitometric analysis of scanned histoblots allowed determination of GluN1 expression in different hippocampal subfields and dendritic layers, delineated with faint dotted lines. The expression of GluN1 was strong in all dendritic layers of the CA1 and CA3 fields and DG. Densitometry data generated at 1 and 6 months of age showed no differences in GluN1 expression in APP/PS1 mice compared to age-matched wild type controls. However, the expression of GluN1 was significantly reduced in the stratum lacunosum-moleculare of the CA1 and CA3 fields and the molecular layer and hilus of the DG of APP/PS1 mice at 12 months of age (Mann-Whitney test, * P < 0,05; ** P < 0,01). Error bars indicate SEM. Abbreviations: CA1 field of the hippocampus; CA3, CA3 field of the hippocampus; DG, dentate gyrus; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum; slm, stratum lacunosum-moleculare; ml, molecular layer; gc, granule cell layer; h, hilus. Scale bars: 0.05 cm