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. 2024 Aug 22;57:56. doi: 10.1186/s40659-024-00537-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Reduced density of synaptic NMDARs in CA3 pyramidal cell-mossy fibre synapses of APP/PS1 mice. Electron micrographs of thorny excrescence (th) of pyramidal cells making excitatory synapses with mossy fibre terminals (mf) in the stratum lucidum of the CA3 field immunolabelled for GluN1, as detected using the SDS-FRL technique in wild type and APP/PS1 mice at 12 months of age. (A-F) Panel A shows a low-magnification image of the P-face of a thick dendrite (Den) of a CA3 pyramidal cell receiving several mf terminals in wild type mice. Electron micrograph in panel B shows a high-magnification image of the boxed area shown in panel A. Immunoparticles for GluN1 were distributed on thorny excrescence (th) of pyramidal cells making excitatory synapses with mf terminals in wild type and APP/PS1 mice. Scale bars: A,D, E,F, 500 nm; B,C, 200 nm. (G) Drawing of a CA3 pyramidal cell, with the blue box delineating the area used for the quantitative analysis in the stratum lucidum (sl). (H) Scatterplots of the number of immunoparticles for GluN1 versus size of excitatory synapses in the stratum lucidum in both wild type and APP/PS1 mice. There is a strong positive linear correlation between immunoparticle number and synaptic size (Pearson’s correlation test). (I) Mean densities of GluN1 in excitatory synapses in thorny excrescences in the hippocampal CA3 field in wild type and APP/PS1 mice. A significant reduction in the density of NMDAR immunoparticles were detected in APP/PS1 mice compared to age matched wild type (n = 3 animals per genotype; unpaired t-test, **P < 0.01)