TABLE 1.
Sites of transposon insertions affecting colony morphology in LM7
| Gene | Frequency | Annotation |
|---|---|---|
| RS00295 | 1 | PAS domain-containing methyl-accepting chemotaxis protein |
| RS00350a | 7 | WcaJ family polyisoprenylphosphate hexose-1-phosphate transferase |
| Upstream RS00360a | 1 | Predicted promoter region of BXU08_RS00360 |
| RS00360a | 4 | Beta-barrel outer membrane porin |
| RS00365a | 2 | Wza family outer-membrane polysaccharide export protein |
| RS00370a | 2 | AAA+ family ATPase |
| RS00375a | 4 | Wzz family polysaccharide chain length determinant |
| RS00380a | 3 | P-loop NTPase |
| RS00385a | 4 | WecG/TagA family glycosyltransferase |
| RS00395a | 5 | Wzy family polysaccharide polymerase |
| RS00400a | 1 | GT2 family glycosyltransferase |
| RS00405a | 1 | GT2 family glycosyltransferase |
| RS00410a | 6 | GT2 family glycosyltransferase |
| RS00415a | 2 | GH10 family glycosylhydrolase |
| RS00420a | 9 | OafA family acyltransferase |
| RS00425a | 4 | GT2 family glycosyltransferase |
| RS00430a | 10 | GH16 family glycosylhydrolase |
| RS00665 | 2 | DedA family membrane protein |
| RS02105 | 2 | PAS domain-containing sensor histidine kinase |
| RS06730 | 1 | Tryptophan halogenase |
| RS07760 | 1 | Type II secretion system protein GspL |
| RS07770 | 1 | Type II secretion system protein GspH |
| UpstreamRS09000 | 1 | Predicted promoter region of BXU08_RS09000 |
| RS09000 | 2 | OmpR family response regulator |
| RS10090 | 1 | Hypothetical protein |
| RS10945 | 1 | Acetoacetyl-CoA reductase PhbB |
| RS11025 | 2 | Poly(R)-hydroxyalkonic acid synthase PhaC |
| RS12915 | 1 | Helix-turn-helix family protein |
| RS13595 | 1 | FliA family transcriptional regulator |
| RS14850 | 1 | Bifunctional (p)ppGpp synthase-hydrolase SpoT |
| RS15820 | 1 | UDP-Galactose 4-epimerase GalE |
| RS18205 | 1 | GT2 family glycosyltransferase |
| RS18930 | 2 | Major facilitator superfamily transporter |
| UpstreamRS19155b | 1 | Predicted promoter region of BXU08_RS19155 |
| RS19155b | 3 | Bifunctional GGDEF-EAL diguanylate cyclase-hydrolase |
| RS19160b | 6 | TPR domain-containing sensor histidine kinase |
| DownstreamRS19160b | 3 | Insertion downstream of BXU08_RS19160 |
| RS19205b | 1 | NAD(P)+-dependent nucleotide sugar dehydrogenase |
| RS19215b | 12 | Amidotransferase, exosortase associated |
| RS19220b | 18 | PLP-dependent aminotransferase |
| RS19225b | 8 | WxcM family acetyltransferase |
| RS19230b | 8 | MviM family dehydrogenase |
| RS19235b | 14 | GT4 family glycosyltransferase |
| RS19250b | 8 | SDR family NAD(P)+-dependent oxidoreductase |
| RS19260b | 12 | WbuX family N-acetyl sugar amidotransferase |
| RS19265b | 10 | AlgZ/HisF2 family acetamidino modification system |
| RS19270b | 9 | HisH family amidotransferase subunit |
| RS19290b | 2 | EpsG family glycosyltransferase |
| RS19295b | 12 | GT4 family glycosyltransferase |
| RS19300b | 2 | Wzy family polysaccharide polymerase |
| RS19310b | 2 | Wzc family tyrosine autokinase |
| RS19360b | 3 | NAD(P)+-dependent epimerase |
a
Insertion sites within the boundaries of Cluster A (promonan).
b
Insertion sites within the boundaries of Cluster B (cell-associated polysaccharide).