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. 2004 Sep 24;383(Pt 1):27–35. doi: 10.1042/BJ20040041

Figure 1. Characterization of the polyclonal antibody Ab2.

Figure 1

(A) Detection of EEN/EA2 protein in human cell lines U937 and HeLa and rodent cell lines NIH3T3 and CHO. The specific signal was completely eliminated after competition of the antiserum with the antigen peptide (right panel). (B) Immunoblot analysis of cell extracts from U937 cells (lane 2) and rodent CHO cells transfected with untagged (CHO+EEN/EA2; lane 1), FLAG-tagged human EEN/EA2 (CHO+F-EEN/EA2; lane 3) or empty vector (CHO+Vector; lane 4) by Ab2 antibody. Endogenous EEN/EA2 protein from CHO cells is indicated by an asterisk.