Figure 4. Involvement of glycosaminoglycans in the interaction between neurocan and syndecan-3 or glypican-1.

(A) ¹²⁵I-syndecan-3 (10000 c.p.m.) was treated with (+) or without (–) heparitinases I and II (Hepase) and then mixed with neurocan–Sepharose treated with (+) or without (–) chondroitinase ABC (Choase). As a control, Sepharose CL-4B was used instead of neurocan–Sepharose CL-4B. After washing, the bound radioactivity was measured with a gamma counter. (B) Binding of neurocan to glypican-1 was examined similarly using ¹²⁵I-glypican-1 (10000 c.p.m.). Results are expressed as the averages for duplicate determinations. Error bars represent the S.E.M.