Table 1. Measurement of the cell-attachment area by centrifugation assay.

The centrifugation assay was performed as described in the Materials and methods section. The diameter (in mm) of the area to which the cells attached was measured as an index of the attachment strength using an internal calibrate graticule. Coating with various substrates and blocking with 1% BSA were performed as described in the Materials and methods section. Results are expressed as the means±S.E.M. for triplicate determinations. *P<0.05, **P<0.001 compared with Mock-N18TG-2 on the recombinant N- or C-terminal neurocan fragment-coated wells. †P<0.01, ††P<0.001 compared with each transfectant on the recombinant N- or C-terminal neurocan fragment-coated wells. FLAG Ab, anti-FLAG monoclonal antibody; N-Neuro, FLAG-tagged recombinant N-terminal neurocan fragment; C-Neuro, FLAG-tagged recombinant C-terminal neurocan fragment; Hep, heparin.

	Diameter (mm)
Substrate	Mock-N18TG-2 cells	Syn3-N18TG-2 cells	Glyp1-N18TG-2 cells
FLAG Ab+BSA	2.77±0.11	2.91±0.10	2.72±0.12
FLAG Ab+BSA+Hep	2.77±0.10	2.96±0.10	2.71±0.07
FLAG Ab+BSA+N-Neuro	3.51±0.10	4.71±0.36*	4.94±0.16**
FLAG Ab+BSA+C-Neuro	3.96±0.08	5.14±0.11**	5.49±0.15**
FLAG Ab+BSA+N-Neuro+Hep	2.92±0.15	3.29±0.16†	3.33±0.11††
FLAG Ab+BSA+C-Neuro+Hep	2.86±0.13	3.10±0.17††	2.92±0.14††