Table 1. Neurite outgrowth in PC12 cells.
Protein concentrations at 100 ng/ml were used unless otherwise stated. ‘+’ denotes neurite outgrowth observed.
| Sample | Neurite outgrowth |
|---|---|
| Mouse NGF (positive control; 100 ng/ml) | +++ |
| B. candidus (250 ng/ml) | ++ |
| N. sputatrix (250 ng/ml) | ++++ |
| A. superbus (250 ng/ml) | ++ |
| P. textilis (250 ng/ml) | +++ |
| Chinese scorpion (250 ng/ml) | ++ |
| Indian scorpion (250 ng/ml) | + |
| Bee venom (250 ng/ml) | ++ |
| Biogel P10 gel fractions of N. sputatrix (Figure 1A) | |
| Fraction 27 | ++ |
| Fraction 28 | +++ |
| Fraction 29 | ++ |
| Fraction 30 | +++ |
| Fraction 31 (1 ng/ml) | +++++ |
| Fraction 33 | ++ |
| Fraction 34 | Lysis |
| Fraction 35 | Lysis |
| Fraction 36 | Lysis |
| HPLC fractions (Figure 1B) | |
| Fraction 3 | − |
| Fraction 4 | − |
| Fraction 5 | + |
| Fraction 6 | ++ |
| Fraction 7 | +++++ |
| Fraction 8 (1 ng/ml) | ++++++ |
| Fraction 9 | +++ |
| Fraction 10 | + |
| Denatured and refolded recombinant NGF | |
| Denatured recombinant NGF (100 ng/ml) | ++ |
| Refolded recombinant NGF (100 ng/ml) | ++++ |