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. 2004 Sep 24;383(Pt 1):159–164. doi: 10.1042/BJ20040265

Figure 1. Binding of SAG to various proteins.

Figure 1

Microplates were coated with serum IgA, secretory IgA (S-IgA) (4 μg/ml), human albumin (10 μg/ml) (A), bovine lactoferrin (1 mg/ml), rabbit IgG antibodies against CEA-1 antigen (10 μg/ml) or gelatin (1 mg/ml) (B). After coating, microplates were incubated with parotid saliva serially diluted in PBST-Ca [1:1 (v/v) starting dilution]. Bound SAG was revealed with antibody 5E9. SAG bound to antibodies, albumin and lactoferrin, but only weakly to gelatin.