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. 2004 Sep 24;383(Pt 1):159–164. doi: 10.1042/BJ20040265

Figure 4. Binding of antibodies to SAG and effect of chemical reduction.

Figure 4

Parotid saliva was separated by SDS/PAGE under reducing (+) or non-reducing (−) conditions, transferred on to nitrocellulose and incubated with antibody 143 against SAG, UEA-1 lectin or alkaline phosphatase-conjugated goat anti-rabbit (goat) or rabbit anti-mouse (rabbit) IgG antibodies. After reduction, the binding of antibody 143 as well as of conjugated antibodies disappeared. With UEA-1 lectin it was observed that SAG migrated more slowly in the gel, suggesting a conformational change.