Fig. 1.

Simultaneous Label-free Autofluorescence Multiharmonic (SLAM) microscopy visualizes label-free signals. a) SLAM microscopy uses a modified ultrafast laser to excite and detect 4 orthogonal endogenous signals. b) THG, 3PF, and 2PF endogenous signals corresponding to sample heterogeneity via THG signal, reduced nicotinamide adenine dinucleotide/reduced nicotinamide adenine dinucleotide (NAD(P)H) signal via 3PF, and flavin adenine dinucleotide (FAD) via 2PF. Signal intensities were collected from 1 field of view of each sample. c) Optically sectioned THG, 3PF, and 2PF signal is generated in organoids across multiple depths denoted by z.