Fig. 2.

SLAM microscopy enables simultaneous visualization of FAD, NAD(P)H, and THG signals to inform prediction of DNA damage response in live samples. a) Brightfield image of NSCLC organoids shows multicellular 3D structures (scalebar: 1000 μm). b) Optical redox ratio (RR) images were generated from ratio of 2 PF FAD signal and 3 PF NAD(P)H signal (scalebars: 50 μm). c) A prediction model for DNA damage response was created by training a gradient boost regression model on linked feature sets of label-free SLAM images and labeled γH2AX foci-stained immunofluorescence images as ground truth.