Fig. 3.

Extracted features and readouts for model training and evaluation. 2D organoid monolayers from a treatment naïve patient were continuously treated with etoposide or bortezomib to generate a training dataset. a) Representative images taken at Day 2 of the NSCLC organoid monolayer using SLAM (3PF, 2PF, THG, RR) and immunofluorescence (DAPI, γH2AX). b) Area under the curve of the redox ratio (AUC RR) is increased relative to blank controls, demonstrating treatment effects on metabolism. c) Mean redox ratio shows a gradual increase then decrease in treatment groups. d) γH2AX foci/nuclei quantification. b,c,d) Statistical testing was performed using ANOVA followed by Tukey's post-hoc test or Kruskal-Wallis followed by Dunn's post-hoc test across all groups within each timepoint. Significance from media control indicated. No statistical differences were detected between media control and DMSO vehicle treatments across all timepoints (3 biological replicates with 3 technical replicates per biological replicate, NS = no significance, *p < 0.05, **p < 0.01, ***p < 0.001).