Fig. 6.

Lack of response in AUC RR feature suggests resistance to DNA damage. 3D HUB047, HUB065, and HUB133 organoids from 3 different treatment naïve patients were cultured in 80 % Matrigel and were continuously treated with 3 μM etoposide for 3 days. a-c) Representative optical redox ratio image at Day 3 in HUB047, HUB065, and HUB133, respectively (scalebar: 50 μm). d) Normalized AUC RR shows differences at Day 3 in HUB047 line but not in HUB065 or HUB133. (4 biological replicates with 9 technical replicates per biological replicate). e) γH2AX foci/nuclei quantification shows statistical significance only in the HUB047 line, mirroring AUC RR results (3 biological replicates with 10–24 technical replicates per biological replicate). d,e) Statistical testing was performed using ANOVA followed by Tukey's post-hoc test or Kruskal-Wallis followed by Dunn's post-hoc test across all groups within each timepoint. Dotted boxes were used to denote shared significance from media control (*p < 0.05, **p < 0.01, ***p < 0.001).