Skip to main content
. 2004 Oct 8;383(Pt 2):209–218. doi: 10.1042/BJ20040794

Figure 2. USF1 and USF2 bind to the HO-1 E-box in vitro by EMSA.

Figure 2

(A) Nuclear extracts from uninduced (vehicle) and haemin (5 μM)-induced HK-2 cells were incubated with a labelled probe containing the HO-1 E-box and surrounding nucleotides (see Table 1 for the probe sequence). USF1 and USF2 polyclonal antibodies were added in samples shown for supershift analysis before the addition of a labelled probe. NRS (normal rabbit serum) is shown as an IgG control. (B) EMSA using the same HO-1 E-box probe as shown in (A) with uninduced and haemin-treated nuclear extracts and anti-c-Myc, -Mad and -Max antibodies. USF1 and USF2 supershifts in (A, B) are indicated by an *. FP, free probe.