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. 2004 Oct 8;383(Pt 2):209–218. doi: 10.1042/BJ20040794

Figure 3. USF1 and USF2 bind to the proximal HO-1 E-box in vivo, but not to an internal E-box.

Figure 3

(A) ChIP of the HO-1 proximal E-box of HK-2 cells induced with haemin or vehicle. PCR for all ChIP assays was performed on immunoprecipitated samples, electrophoresed, transferred on to a nylon membrane and subsequently hybridized with a nested oligonucleotide as described in the Experimental section. (B) ChIP of primary HPTCs treated with haemin (H, 5 μM), cadmium chloride (Cd, 10 μM) or vehicle (V). (C) ChIP performed on the human HO-1 internal E-box located in intron 2. (D) DMS in vivo footprinting of the HO-1 internal E-box-transcribed strand. The E-box and flanking sequences are illustrated.