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. 2004 Oct 8;383(Pt 2):209–218. doi: 10.1042/BJ20040794

Figure 4. USF2b is the predominant form of USF2 expressed in HK-2 cells.

Figure 4

(A) Total RNA was purified from vehicle or haemin-treated HK-2 cells, then reverse-transcribed and used as template for PCR. Primers used (see the Experimental section) flank the fourth exon of human USF2. PCR products lacking the fourth exon (USF2b) and containing the fourth exon (USF2) are shown. Sizes of the DNA ladder are indicated in bp. (B) Western-blot analysis of HK-2 cells transiently transfected with USF2, USF1 or empty vector. Anti-USF2 antibody confirms USF2 overexpression (mass, approx. 44 kDa; lane 1). Endogenous USF2- (marked as USF2b) in the USF1-(lane 2) and empty vector- (lane 3) transfected cells is recognized as a lower-molecular-mass (approx. 38 kDa) protein.