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. 2004 Oct 8;383(Pt 2):209–218. doi: 10.1042/BJ20040794

Table 1. Sequences of oligonucleotide (oligo) primers.

Primer type Sequence (all listed as 5′→3′)
In vivo footprinting
 Linker adapter (equimolar amounts) GCGGTGACCCGGGAGATCTGAATTC (also used in LMPCR) and GAATTCAGATC
 For the non-transcribed strand
  Extension oligo CAAGCAGTCAGCAGAGGATT
  PCR and hybridization oligo TGGCCAGACTTTGTTTCCCAAGGGTCA
 For the transcribed strand
  Extension oligo GTAAATTACCGTTCCTCCCT
  PCR and hybridization oligo TCGGGTTGCGGACGCTCCA
EMSAs
  Forward CTGTTCCGCCTGGCCCACGTGACCCGCCGAGCATAA
  Reverse TTATGCTCGGCGGGTCACGTGGGCCAGGCGGAACAG
Site-directed mutagenesis
 pHOGH/4.5 E-box M1
  M1 forward CTGTTCCGCCTGGCCCACGTGAtCCGCCGAGCATAA
  M1 reverse TTATGCTCGGCGGaTCACGTGGGCCAGGCGGAACAG
 pHOGH/4.5 E-box M2
  M2 forward CTGTTCCGCCTGGCCCAtGTGACCCGCCGAGCATAA
  M2 reverse TTATGCTCGGCGGGTCACaTGGGCCAGGCGGAACAG
 pHOGH/4.5 E-box M3
  M3 forward CTGTTCCGCCTGGCCtACGTGACCCGCCGAGCATAA
  M3 reverse TTATGCTCGGCGGGTCACGTaGGCCAGGCGGAACAG
 pHOGH/4.5 Δ E-box
  Del E-box F CATCAGCTGTTCCGCCTGGCCGCCGAGCATAAATGTG
  Del E-box R CACATTTATGCTCGGCGGCCAGGCGGAACAGCTGATG
ChIP
 For the proximal E-box
  Forward TTGCAACGCCCGGCCAGAAA
  Reverse TCGGGTTGCGGACGCTCCA
  Nested hybridization oligo ATAAATGTGACCGGCCGCGGCTC
 For the internal E-box
  Forward GATGACACTGAGGCTCAGA
  Reverse GCTGAAATCGGTCTTGGTTGATTTCAGCC
  Nested hybridization oligo GGTCACACAGTAAGTTCAGCCTGCTCT