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. Author manuscript; available in PMC: 2024 Aug 22.
Published in final edited form as: Sci Transl Med. 2022 Jun 22;14(650):eabo5032. doi: 10.1126/scitranslmed.abo5032

Figure 2. DS-Cav1 immunization activates pre-F and dual IgG+ B cells.

Figure 2.

(A) Representative flow cytometry plots from a single participant that received two immunizations show RSV pre-F-binding (red), dual-binding (purple), and post-F-binding (blue) IgG+ B cells overlaid on top of total IgG+ memory B cells (gated on mononuclear cells/singlets/live/CD19+/CD20+CD38−/IgD−/IgG+/CD27+). The populations were defined based on their expression of CD21 (downregulated upon activation) and CD71 (proliferation marker). The frequencies of RSV pre-F-binding and dual-binding IgG+CD27+ B cells were quantified in individuals who received (B) one (n=33) or (C) two (n=62) immunizations. Probe-binding frequencies are normalized to 100%.