Table 2. ER-targeted RTB blocks neither binding nor endocytosis of exogenously added RTB.
Endocytosis assay | |||
---|---|---|---|
Scatchard analysis | |||
Affinity (Kd, μM) | Binding sites/cell (×106) | Uptake of [125I]RTB (% relative to control cells) | |
HeLa/LacZ | 1.17 (±0.04) | 91.9 (±9.5) | 100 |
HeLa/RTB-H2 | 1.19 (±0.02) | 87.9 (±3.3) | 87.7 (±2.4) |