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. 2004 Oct 8;383(Pt 2):327–334. doi: 10.1042/BJ20041062

Figure 4. IgE reactivities to recombinant hazelnut allergens determined by Western blotting.

Figure 4

Separation of rCor a 1.04, rCor a 2 and rCor a 11 by SDS/PAGE (a selection of 10 out of 65 patients; NC, negative control). All sera shown were positive to rCor a 1.04, whereas patient 4 and 8 were also positive to rCor a 2. Four patients showed positive IgE reactivity to rCor a 11 (sera 1, 3, 5 and 10). Sera 3, 6, 8 and 10 were used in histamine-release assays. IgE binding to rCor a 8 was assayed in a separate experiment under non-reducing conditions and was negative for all patients (results not shown).