Table 3. Selected sera used in histamine release assays.

		Hazelnut extract	rCor a 1.04	nCor a 11			rCor a 11
Serum number	Experiment…	HR†	HR	ELISA‡	Blot	HR	ELISA	Blot	HR	CCD ELISA [22]
1*		0.2	1×10−4	0.8	+	8	0.3	+	6	0.48
2*		0.3	1×10−4	2.3	++	5	1.3	++	5	0.55
3		0.3	1×10−4	0.5	+	2	0.4	+	3	0.15
4		0.5	2×10−4	0.4	+	2	0.3	+	5	0
5		0.3	nd	0.77	++	5	0.3	+	3	0.96
6		0.2	8×10−4	0.43	+	10	0.3	+	10	0.78

* DBPCFC positive.

† HR results are expressed as allergen concentration eliciting mediator release of 25% of the total histamine release from passively sensitised basophils in μg/ml. No histamine release was observed with the non-allergic control. Nd, not determined.

‡ Considered as positive with an absorbance of at least 0.3 (3-fold of the negative control).