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105 bp DNA was incubated with T4 DNA ligase for 60 min in the presence of 2 pmol of HBsu (lane 2) or 0–7 pmol of HpyHU respectively (lanes 3–10). The reaction mixture in lane 1 contained no ligase. Less than 10% of total DNA is cyclized at the highest [HpyHU]. HpyHU-RN (1–7 pmol) does not cyclize the 105 bp DNA (lanes 12–18) above that seen in absence of HU (lane 11). DNA was resolved on an 8% polyacrylamide gel.
