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. 2004 Nov 9;384(Pt 1):59–68. doi: 10.1042/BJ20040427

Figure 2. SDS/PAGE analysis of the purified enzymes.

Figure 2

Purified proteins were resolved by SDS/10% PAGE. The first lane contains molecular-mass markers (sizes indicated in kDa), while the remaining lanes include the purified proteins designated in Figure 1 as wild-type tobacco AHAS (lane 1), AHAS Δ630 (lane 2), AHAS Δ567–582 (lane 3), AHAS Δ567 (lane 4) and AHAS Δ598 (lane 5). These enzymes were overexpressed as N-terminal GST-fusion proteins and purified by the affinity resin, glutathione–Sepharose. In addition, GST-free AHASs were generated by the treatment of thrombin described in the Experimental section and were analysed on the same SDS/PAGE gel in lane 6 (wild-type AHAS; 66 kDa), lane 7 (AHAS Δ630; 62 kDa), and lane 8 (AHAS Δ567–582; 64 kDa).