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. 2004 Nov 9;384(Pt 1):149–157. doi: 10.1042/BJ20040928

Figure 6. Activation of PPARγ by curcumin blocks TGF-β signalling in activated HSC.

Figure 6

(A) Passaged HSC were transiently transfected with the TGF-β-inducible luciferase reporter plasmid p3TP-Lux. Cells were then pretreated with or without PD 68235 (20 μM) prior to the addition of curcumin (Cur.) at the indicated concentrations for an additional 36 h. Luciferase activities are expressed in relative units after β-galactosidase normalization (n=6). Significance: *P<0.05 compared with cells without curcumin; **P<0.05 compared with cells treated with 20 μM curcumin. (B) HSC in 6-well culture plates were co-transfected with a total of 4.5 μg of plasmid DNA per well, including 2 μg of p3TP-Lux, 0.5 μg of pSV-β gal, pPPARγcDNA at the indicated doses and an empty vector. The amount of DNA in pPPARγcDNA plus the empty vector was 2 μg. Cells were then treated with or without 20 μM curcumin for 36 h. Luciferase activities are expressed in relative units after β-galactosidase normalization (n=6). Significance: *P<0.05 compared with cells transfected with no pPPARγ cDNA and not treated with curcumin.