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. 2004 Nov 9;384(Pt 1):149–157. doi: 10.1042/BJ20040928

Figure 8. Curcumin inhibits the expression of TGF-β receptor genes in passaged HSC, which requires the activation of PPARγ.

Figure 8

Passaged HSC were pretreated with or without the specific PPARγ antagonist PD 68235 (10 or 20 μM) for 30 min prior to the addition of curcumin (Cur.; 20 μM) for an additional 24 h. Total RNA and whole-cell protein extracts were prepared. (A) Real-time PCR of the TGF-β receptors Tβ-RI and Tβ-RII. GAPDH was used as an invariant control for calculating fold changes in mRNA levels (n=3). Values are expressed as means±S.D. Significance: *P<0.05 compared with cells with no treatment; †P<0.05 compared with cells treated only with curcumin. (B) Western blotting analyses of Tβ-RI and Tβ-RII. β-Actin was an internal control for equal loading (n=3).