Table 1. Proteins associated with Stauf1-containing complexes.
Note that EF1α was not detected as a component of Staufen RNPs, either via MS or Western blotting, and served as a negative control. The band sizes (kDa) shown in the final data column were as seen in the gel. Explanation of synbols: +, detected; −, not detected; n.d., not determined.
| Protein | GenBank® accession no. | MS | Western blot | Band size (kDa) |
|---|---|---|---|---|
| RNAH | NP_076950 | + | n.d. | 150 |
| hnRNPU (SAFA) | NP_114032 | + | + | 120 |
| NFAR | Q12906 | + | n.d. | 110 |
| Kinesin heavy chain | A41919 | − | + | 110 |
| Nucleolin | NP_005372 | + | + | 105 |
| PABP | P11940 | − | + | 75 |
| FMRP | Q06787 | − | + | 75 |
| Dynein intermediate chain | O14576 | − | + | 70 |
| β4-Tubulin | Q13509 | + | n.d. | 50 |
| α-Tubulin | P05209 | − | + | 50 |
| EF1α | CAA34756 | − | − | 50 |
| Ribosomal protein P0 | NP_000993 | + | + | 34 |
| Ribosomal protein L6 | AAH20679 | + | n.d. | 34 |
| Ribosomal protein S2 | NP_002943 | + | n.d. | 32 |
| Ribosomal protein S6 | P10660 | + | + | 32 |
| Ribosomal protein S3a | L13802 | + | n.d. | 30 |
| Ribosomal protein L7a | NP_000963 | + | + | 30 |
| Ribosomal protein S8 | S11415 | + | n.d. | 28 |
| Ribosomal protein L14 | P50914 | + | n.d. | 27 |
| Ribosomal protein L10 | S34425 | + | n.d. | 26 |
| Ribosomal protein L15 | P61313 | + | n.d. | 26 |
| Ribosomal protein L21 | S55913 | + | n.d. | 20 |