Table 1. Apparent molecular masses from light scattering analyses of full-length ROCK II and the four ROCK I constructs.
The theoretical molecular mass of each construct was calculated using the ProtParam Tool available on the ExPASy Molecular Biology Server (www.expasy.org).
| Molecular mass (Da) | |||
|---|---|---|---|
| Construct | Theoretical | Apparent | Ratio (apparent/theoretical) |
| Full-length ROCK II | 161308 | 426000±21300 | 2.6 |
| S6–L553 | 64210 | 164066±5400 | 2.6 |
| S6–R415 | 47840 | 100467±153 | 2.1 |
| S6–E379 | 43705 | 54490±220 | 1.2 |
| M71–E379 | 35995 | 36800±630 | 1.0 |