Table 3. Summary of parameters obtained from analyses of the sedimentation equilibrium data.
Molecular mass (Da) | ||||
---|---|---|---|---|
Construct | Apparent | Ratio(apparent/theoretical) | Model used for data fitting | Kd (μM) |
Full-length ROCK II | 318658±20828 | 2.0 | Single species | – |
S6–L553 | 132093±6713 | 2.1 | Single species | – |
S6–R415 | 91257±4230 | 1.9 | Monomer/dimer | 0.4 |
S6–E379 | 57618±4230 | 1.3 | Monomer/dimer | 17.6 |
M71–E379 | 38394±460 | 1.1 | Monomer/dimer | 357.0 |